E-49 キュウリのオーキシン応答性遺伝子CsPK3シロイヌナズナ相遺伝子APK100のクローニング及び解析(2)

概要

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CsPK3 is an auxin and gibberellin-responsive protein kinase gene from cucumber hypocotyls, and its expression is down regulated by light irradiation. The precise expression analysis suggested the involvement of CsPK3 in the shoot elogation. Considering the use of mutant plants for further analysis of the gene function, we performed the cloning of its Arabidopsis homolog. The cDNA showed an ORF (476 A.A.) with high similarity with CsPK3 (78%) , possessing all the conserved amino acids characteristic of serine/threonine protein kinases and the N-terminal domain rich in serine and threonine residues. Northern blot analysis indicated that the expression of this gene rapidly decreased by light irradiation. The effects of the plant hormones on the expression are under examination.
植物化学調節学会の論文
1998-10-01