D-41 アサガオ未熟種子における活性型ジベレリンの免疫組織化学

概要

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Young developing seeds of several dicotyledonous plants contained a large amount of gibberellins (Gas). Their physiological roles, however, remain unknown. Their localization and fluctuation may give informations about their roles in seed development. We focused on the localization of GAs, especially so-called "active GAs" in developing seeds of Pharbits nil with an immunohistochemical technique. To avoid the drift of GAs during their fixation, we adopted the combination of lyophilization and fixation using gaseous diisopropylcarbodiimide. Seeds clipped at various developing stages were subjected to the fixation process, embedded in paraffin, sliced into sections of 10 micrometer thick, and stained with an antiserum specific for 7-substituted GA_1. The staining was visualized with the streptavidin-biotin peroxidase system. The specific staining due to active GAs was observed in outer-integument and immature cotyledons of seeds. At higher magnification, many starch grains were observed in outer-integument. GAs were localized at an antipole of the hilum of the starch grains. The relationship between the transition of this localization of GAs and the metabolism of the starch grains will be discussed.
植物化学調節学会の論文
1998-10-01