E-48 オーキシン応答性タンパク質リン酸化酵素遺伝子CsPK3の機能解析

概要

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We previously cloned the cDNA of an auxin (IAA)-responsive protein kinase gcne named CsPK3from cucumber. On PCR and subsequcnt 5'-and 3'-RACE, we two tobacco homologs (NtPK3I and NtPK3II) of CsPK3. To examine the biological function of CsPK3, we made transgenic tobacco plants overexpressing CsPK3 and its tobacco homologs. CsPK3 cDNAs were fused to cauliflower mosaic virus 35s promoter in sense and antisense direction, and the chimeric genes were intoroduced intotobacco. The overexperssion of CsPK3 and its tobacco homologs in sense and antisense orientation did not show any obvious phenotypic changes in transgenic tobacco. Then, we screened cucumber genomic DNA library to clone CsPK3 genomic DNA. The transgenic tobacco plants have bein generated that cxpress the β-glucuronidasc (GUS) gene under the control of the CsPK3 promoter. The GUS expresson was detected in the shoot apical meristem, leaf primordia, epidermis and vascular tissues. The CGU staining in rcsponse to IAA was observed in the hypocotyl of transgenic plants. The GUS expression was high in the dark-grown seedlings, and decreased after irradiation with white light
植物化学調節学会の論文
1998-10-01