16.シロイヌナズナにおけるジベレリン受容体遺伝子多重欠損変異株の解析(口頭発表)

概要

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Gibberellin (GA) participates in many aspects of plant growth and its development. Following the identification of rice GA receptor gene (OsGIDl), we also found three receptor genes (AtGIDla, AtGIDJb, AtGIDl c) in Arabidopsis. Each recombinant protein of the AtGIDl gene showed a high affinity to biologically active GAs, and the expression of each AtGIDl gene could complement the dwarfed phenotype of rice gidl-1 mutant. In this study, we elucidated the functional redundancy and specificity of each AtGIDl by using some T-DNA or Ds insertion mutants. The phenotype of the homozygous single knock-out mutants (that is, atgidla-1KO, atgidlb-1KO, or atgidlc-1KO) are all similar to that of wild type plants while we found distinctive phenotypes in 2KO mutauts: (i) the atgidla atgidlb-2KO showed shorter stamens, and then low fertileity and (ii) the atgidla atgidlc-2KO showed a dwarfed phenotype. Moreover, it was observed that the atgidla atgidlb atgidlc-3KO mutant did not germinate, however it began to grow after the removal of seed coat. Its seedlings showed a severely dwarfed phenotype, and a complete loss of their ability to respond to GA. Taken together, we concluded that three AtGIDls function as GA receptors in Arabidopsis with high redunduncy, but partially have specific role(s) for growth and development of Arabidopsis.
植物化学調節学会の論文
2007-10-05