12.ジベレリンシグナル伝達制御剤の探索[I] : 代謝酵素阻害剤(口頭発表)

概要

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The hydroxylation of gibberellin (GA) by GA 2-oxidase (GA2ox), a 2-oxoglutarate dependent dioxygenase, is known to be one of its major inactivations. The regulation of this catabolic step is very important for a fluctuation of endogenous GA in plants, and therefore is a good chemical target to control the gibberellin action. At present, prohexadione is known as a commercially available inhibitor to GA2oxs, however prohexadione has a characteristic that it inhibits other 2-oxoglutarate dependent dioxygenases like GA 3-oxidases. In this report, we performed in vitro random screenings to obtain novel inhibitors more specific to GA2oxs, by using radio-labeled GA_4-derivative and recombinant Arabidopsis GA2ox2. As a result, we discovered an inhibitory effect of methyl 6-chloro-3H-1,2,3-benzodithiazole-4-carboxylate 2-oxide (CBTC) to the enzymatic activity of GA2ox2, and we subjected it to the successive in planta evaluations. The CBTC promoted both the germination of Arabidopsis seeds and their growth. This strongly suggests that CBTC specifically inhibits GA2oxs, not GA3oxs, in Arabidopsis.
2010-10-01