70.ブラシノステロイド情報伝達突然変異体のFOXハンティング変異体群からの探索(口頭発表)

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We had identified several brassinosteroid signaling mutants from EMS-mutation lines, fast neutron-mutation lines and activationtag lines in Arabidopsis. To identify new brassinosteroid signaling mutants, we used FOX (Full-length cDNA over-expressor gene) hunting system mutants, which were overexpressed mutants of a normalized Arabidopsis full-length cDNA library. For these screening, we used Brz that is an inhibitor of brassinosteroid biosynthesis enzymeDWF4. Brz treatment caused to wild type as shorten hypocotyls in the dark and highly dark green leaves in the light. We tried to screen about 8000 Fox-lines and isolated three type mutants. At first we screened mutants that shows type was showed longer hypocotyls than wild type with Brz in the dark as bil (Brz-insensitive-long hypocotyls) and mutants that showed shorten hypocotyls than wild type with Brz in the dark as bss (Brz-sensitive-short). Furthermore, we screened mutants that showed pale green leaves in spite of growing on the Brz in the light as bpg (Brz-insensitive-pale green). Further analyses are in progress.
2010-10-01