2.IAA生合成阻害剤を用いた変異体の探索(口頭発表)

概要

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Plant hormone indole-3-acetic acid (IAA) has various physiological functions, but IAA biosynthetic and signal pathway is still unclear. As IAA is essential for plant growth, isolation of knock-out mutants in the main pathway of IAA biosynthesis and the isolation of double mutants in the knock-out mutant background may have been difficult. It has been suggested that there should be several pathways in IAA biosynthesis and some of them may function complementarily to each other. Therefore mutants that have defect in such pathway may show no significant phenotype to distinguish the mutants from wild type. If we have IAA biosynthesis inhibitor targeting the main pathway of IAA biosynthesis, it would make it possible to isolate new mutants because such inhibitors can bypass the difficulties described above by controlling the concentrations at which they are used. That is, the plants can grow with altered phenotypes under chemical treated-conditions, and we can isolate double or triple mutant-like mutants by the mutant screening with the inhibitors. Our aim of this study is the isolation of new IAA mutants (rai: resistance to auxin inhibition) from mutanized seeds of Arabidopsis by using IAA biosynthesis inhibitor, L-2-aminooxy-3-phenylpropionic acid (L-AOPP). At present we isolated one.
2010-10-01