光形態形成および葉緑体分化におけるブラシノステロイド作用の分子機構(奨励賞,受賞業績)

概要

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Chloroplast differentiation was tightly related with photomorphogenesis that based on hypocotyls elongation and cotyledon opening. At first, to analysis mechanism of chloroplast differentiation, we cloned two regulator of chloroplast genome, CND41 and PTF1. At the second, we identified that brassinosteroid deficiency in the dark with Brz, brassinosteroid biosynthesis inhibitor, caused not only the deetiolation but also the acceleration to chloroplast from etioplast after the short-term-light treatment to cotyledon that germinated in the dark with Brz. Many chloroplast genes that were encoded on the both of nuclei and plastid genome were expressed in the dark with Brz higher than the control plant without Brz. In order to analyze in detail the mechanisms of brassinosteroid signal transduction, we screened for mutants that showed longer hypocotyls than wild type when grown with Brz in the dark, and designated bil mutants (Brz-insensitive-long hypocotyl). We identified a dominant mutant bill that encoded on a novel bHLH trans-acting factor that stability was regulated by a kinase and proteasome system.
植物化学調節学会の論文
2007-05-31