36.ブラシノステロイド情報伝達突然変異体bpg2、bss1の原因遺伝子の葉緑体制御・発生制御における機能の解析(口頭発表)

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We tried to screen new brassinosteroid signaling mutants from Arabidopsis activation tagging lines and isolated two mutants. In the light, Brz treated plant showed dark green leaves as compared to non-treated leaves. bpg2 (Brz-insensitive pale green 2) was screened as pale green phenotype with Brz in the light. A T-DNA insertion site of bpg2 mutant was identified in an ORF of novel GTPase homologous gene that were conserved in many organisms. Expression of BPG2 gene was induced by light and Brz, and was highest in greening organs. BPG2-GFP fusion protein analysis revealed that BPG2 located in chloroplasts. In bpg2 mutant, abnormal chloroplast rRNA processing and protein accumulation were observed. These results might suggest that BPG2 plays an important role of posttranscriptional and/or translational regulation for chloroplast genome on brassinosteroid signaling. The semidominant mutant bss1 (Brz-sensitive-short1) showed normally etiolated hypocotyl same as wild type without Brz in the dark. However, with Brz, bss1 hypocotyl became about 50% length of wild type in the dark. In the weak light, hyocotyl of bss1 showed about 50% length of wild type without Brz. These results suggested that bss1 hypocotyl is hypersensitive to Brz and the light. In the later growth stage, bss1 showed extremely dwarf phenotype by inhibition of axis elongation. An activation tag insertion was identified in non ORF region on bss1 genome. Further analysis is in progress.
植物化学調節学会の論文
2008-10-06

36.ブラシノステロイド情報伝達突然変異体bpg2、bss1の原因遺伝子の葉緑体制御・発生制御における機能の解析(口頭発表)

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