P-43 メロンがんしゅ病菌Streptomyces sp. CB-1-1株の自己胞子発芽抑制物質(ポスター発表の部)

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Root tumor of melon was first found in 1982 in Japan. This diseese is characerized by roots developing many galls, and growth-retardation and wing in the above-ground parts of plants, The pathogen of this diseese is a Streptomyces, which is thought to be unrelated to previousty descrbed plant-pathogenic Streptomyces species. About 10-20% spores of the pathogen, Streptomyces sp. B-9-1, were ungerminated spores, A heat-shock treatment increased the colony forming rate to 110-115% of the untreated contral, and it was more effective in SDS solution. These stirmutative effects suggested that the spores contained a factor that inhibited their own germination. Studies of self-inhibitors are of interest in understanding the regulatory mechanisms of spore germination, and will lead to the development of new techniques that prevent or manage diseases. We isolated a spore germination inhibitor from the liquid-cultured material of Streptomyoces sp. B-9-1, the causal organism of root tumors in melon, and idernffied it as anthranrific acid. However, anthranfic acid showed an IC_<50> at ca 50μg/ml, and its content in spores was very few, indicating that its contribution to the inhibition of spore germination was smal. Reinvestigation of germination inhibitor led us to find a new inhibitor in the spore extract of another strain CB-1-1 (yield, 0.25mg from 3,500 Petridishes). The inhibitor had a characteristic UV spectrum (λ_<max> 263, 318nm), The molecular formula of the inhibitor was determined to be C_7_H_7N_3O_3 by high resolution LC-ESHVIS. In-source CID-MS analysis of the inhibitor and reaction product of the inhibitor with CH_2N_2 led to the fact that the inhibitor contained carboxyl group and acetyl group. The possible compounds, 2-amino-β-axopyrazinepropanoic acid and 3-acetylaminopyrazine-2-carboxylic acid, were selected based on these structural characteristics and UV spectrum specutated by computational chemistry. The possible compounds were synthesized, and were compared with the inhibitor by LC-MS analysis and the inhibitor was determined to be 3-acetylaminopyrazine-2-carboxylic acid. Moreover, we confimed that the inhibitor was produced by other Streptomyces spp., S. coeticolor A3(2) and S. griseus NBRC3237, and we consider the possibility that the inhibitor is a common regulator of spore germination in Streptomyces.
天然有機化合物討論会の論文
2007-08-24

P-43 メロンがんしゅ病菌Streptomyces sp. CB-1-1株の自己胞子発芽抑制物質(ポスター発表の部)

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