62. シロイヌナズナを用いた新規2-デオキシ-7-オキサラクトン型ブラシノステロイドの生合成研究

概要

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The natural occurrence and biosynthesis of 2-deoxy-7-oxalactone brassinosteroids (BRs) in Arabidopsis thaliana were studied. In GC-MS analysis, 7-oxateasterone (7-OXTE) and 7-oxatyphasterol (7-OXTY) were identified from wild type (Ws2) and BR-insensitive mutant bri1-5, respectively. To demonstrate the metabolism of 7-OXTE to brassinolide (BL), feeding experiments of 7-OXTE and 7-OXTY into det2-1, which is BR-deficient mutant and lacks endogenous BRs, were examined. The conversion from 7-OXTE to 7-OXTY was determined by GC-MS. Although no reversible conversion from 7-OXTY to 7-OXTE occurred, the production of BL from 7-OXTE and 7-OXTY has not been determined yet. Both 7-OXTE and 7-OXTY promoted hypocotyl elongation of det2-1, and the activity of 7-OXTY showed higher than that of castasterone. These results suggest that a new lactonization pathway of BL biosynthesis may be operated in higher plants.
植物化学調節学会の論文
2005-10-13