45(P-9) 含窒素ポリケタイド・パママイシンの生合成研究(ポスター発表の部)

概要

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Pamamycin (PM, 1) is an aerial mycelium inducer isolated from Streptomyces alboniger. It is a unique sixteen-membered macrodiolide with a dimethylamino group-bearing side chain and dimethylamino group of PM is indispensable for the activity. Feeding experiments with <13>^C-labeled acetate and propionate, or <15>^N-labeled precursor showed that the carbon skeleton of PM-607 (MW 607) was assembled from six acetate, four propionate and three succinate units, and the nitrogen atom was derived from α-amino group of amino acid. We also revealed that two N-methyl groups of PM were derived from methionine by feeding experiment with [methyl-2^H_3]-methionine. Analyses of these intermediates accumulated in the blocked mutants of PM biosynthesis showed accumulation of hydroxy acids 3, 4 and 5, and the absence of ketone intermediate 2. These results indicated that the transamination occurred in keto-hydroxy acid 3 to generate 5. Quantitative analyses of PM intermediates 3, 4, 5 and PM in the cultured materials of parent strain indicated that transamination was the rate-determining step in PM production.
天然有機化合物討論会の論文
2004-10-01