11. Gibberella fujikuroi交配群のジベレリン生合成に関する化学的および遺伝学的分析

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As well as being a phytohormone in higher plants, gibberellins (GAs) are secondary metabolites of Gibberella fujikuroi as the rice bakanae disease fungus. G. fujikuroi is a complex of at least nine different mating populations (MP-A〜I). The bakanae disease caused by MP-C, which produces GA_3. Other MPs, however, have been not recognized as GA producers. We report here the chemical and molecular analyses for GA production and the biosynthesis in G. fujikuroi MPs. GC-MS analysis of mycelial extracts from six MPs (A〜F) revealed that ent-kaurene, an intermediate of fungal GA biosynthesis, was identified from all MPs. Elongation activity of Chinese cabbage hypocotyls was shown in EtOAc-soluble fractions extracted of the culture filtrate from MP-C and MP-F. From the GC-MS analysis of these fractions, GA_<1/3> and GA_1 were identified from MP-C and MP-F, respectively. We also examined PCR experiments for amplification of fungal GA biosynthesis-related enzyme genes from the genomic DNA. Each genomic DNA was prepared from the six MP strains and used as a DNA template. To amplify DNA fragments of HMG-CoA reductase, fungal ent-kaurene synthase, and P450s involving in fungal GA-biosynthetic enzymes, degenerate DNA primers were designed from conserved amino acid regions of each enzyme. DNA fragments of HMG-CoA reductase and P450s were successfully amplified from all samples, but each ent-kaurene synthase gene was detected as smear band. The sequence analysis of amplified DNAs are also in progress.
植物化学調節学会の論文
2004-10-13

11. Gibberella fujikuroi交配群のジベレリン生合成に関する化学的および遺伝学的分析

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