30. LC/MS/MSを用いた植物中のPQQの分析

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Pyrroloquinoline quinone (PQQ), an element discovered as a novel cofactor in bacterial dehydrogenase, has been identified as an important player in mammals and now recognized as a new vitamin. PQQ is reported to be in common foods, such as meat, fruits, vegetables, and crops with HPLC or GC/MS analyses, but the quantitative analysis of PQQ has been difficult because it readily forms hydrate and/or adducts with nucleophilic reagents such as amino acids. Here, we report a method for quantifying various forms of PQQ and its derivatives in plants. This methods will be an important tool for solving a question whether PQQ is synthesized in plants or not, and how plants synthesize PQQ. Multiple reaction monitoring (MRM) of LC/MSMS was used for that purpose because this system has an advantage over the traditional LC or GC/MS in that MRM has a shortened analysis time and can eliminate the need for a derivatization step. We used Arabidopsis thaliana grown in germ-free environments on MS agar plates as our sample. The results demonstrated that PQQ and its various derivatives exist in Arabidopsis. To date, it has long been thought that only bacteria biosynthesize PQQ, but above result could suggest that higher plants can synthesize PQQ. Highly homologous genes with bacterial PQQ metabolic enzyme genes had not been identified in Arabidopsis genome yet, but a new analytical method for PQQ described above will allow us to uncover PQQ biosynthesis and function in plants.
2005-10-13

30. LC/MS/MSを用いた植物中のPQQの分析

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