5.オーキシン生合成阻害剤候補化合物を用いたIAA変異体の探索(口頭発表)

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Plant hormone indole-3-acetic acid (IAA) has various physiological function, but IAA biosynthetic and signal pathway is still unclear. IAA is essential for plant growth, therefore the analysis of IAA biosynthesis knock-out mutants in the main pathway and the selection of double mutant with the knock-out mutant background may have been difficult. There are several pathways in IAA biosynthesis and some of them may function complementarily to each other. Mutants that have defect in such pathway may show no significant phenotype to distinguish mutants from wild type. On the other hand, the use of IAA specific biosynthesis inhibitor may bypass these difficulties and would make it possible to isolate new mutants. Screening of FOX hunting line with the inhibitor is also the powerful combination to find new mutants. We can raise the next point as an overall merit of using IAA biosynthesis inhibitor for isolating IAA mutants: we could isolate double or triple mutant-like mutants by the screening mutants with the inhibitor. Our aim of this study is the screening of mutanized seeds of Arabidopsis by using IAA biosynthesis inhibitor, L-2-aminooxy-3-phenylpropionic acid (L-AOPP) and isolate new IAA mutants (Resistance to Auxin Inhibition: RAI). At present we isolated one mutant that shows no significant phenotype without the treatment of L-AOPP. Therefore it is clear that use of chemicals for mutant screening has an advantage over the conventional mutant screening.
2009-10-06

5.オーキシン生合成阻害剤候補化合物を用いたIAA変異体の探索(口頭発表)

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