40.アシル化スペルミジン誘導体処理が誘導するイネ病害抵抗性反応(口頭発表)

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Spermidine (Spd) is a well-known plant polyamine involved in disease resistance responses, especially hypersensitive response (HR). HR is a powerful resistance system plants have developed against pathogen attack, occurred with production of reactive oxygen species including hydrogen peroxide (H2O2). It is already reported that, H2O2 is generated during Spd oxidation by polyamine oxidases in plant and the induction of HR by Spd infiltration into tobacco is suppressed with guazatine, a polyamine oxidase inhibitor, but the reactive mechanism of HR still remains unclear. Here we synthesized some Spd derivatives to examine the effect of this chemical modification on disease resistance responses, and compared their inductive activities with that of Spd to investigate the possibility if Spd derivatives induce disease resistance more efficiently. The target amino group is definable with using appropriate protective group, and Spd is easily acylated with acylchloride. Thus, we synthesized some Spd mono acyl derivatives. We prepared 1mM solution of them including 0.1% tween20, then treated them as 5μl drop on leaf blades of 4 week soil-cultured rice (nihonbare). As a result, we found some Spd derivatives which caused HR-like cell death more efficiently than Spd. Now we examine the effects of them using real-time PCR on the expression of OsPR1b and PBZ1, representative resistance marker gene of rice, and recently discovered WRKY45, a resistance gene elevated by benzothiadiazole.
2010-10-01

40.アシル化スペルミジン誘導体処理が誘導するイネ病害抵抗性反応(口頭発表)

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