78. イネアクチベーションタギングにより単離された擬似病斑変異体及び短粒半わい性変異体の解析

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We have generated 12,000 activation-tagging lines of rice. In these lines, a T-DNA that contains tetramer of the CaMV 35S enhancer was introduced. Among them, several mutants including lesion mimic, stripe, dwarf, and short grain were obtained. A lesion mimic mutant, which we designated Lesion mimic 1 (Lmm1), was further characterized. Lmm1 was dominant and the phenotype linked to the T-DNA insertion. OsACT1 whose encoded protein shows sequence similarity to an acyltransferase was located about 500 bp downstream of the T-DNA insertion. In addition, transcriptional levels of PR proteins were increased, and phytoalexins, both momilactone A and sakuranetin, accumulated in Lmm1. We previously reported these at the JSCRP meeting in 2003. This year, we indicated that the Lmm1 mutant showed resistance to fungal blast. Further, we combined OsACT1 genomic DNA downstream of the 35S promoter and re-introduced it into rice. The lesion mimic phenotype was detected in the produced transgenic lines, which clearly indicates that the overexpression of OsACT1 caused Lmm1 phenotypes. Next we analyzed the Short grain 1 (Sg1) mutant. When the T-DNA insertion is heterozygous, the grain is small, and when it is homozygous, the grain is very small, indicating that the Sg1 phenotype is linked to the T-DNA insertion. The Sg1 plant also shows semi-dwarf phenotype. Gibberellin restored the semi-dwarf phenotype of Sg1. A full-length cDNA lies 1.4kb downstream of the inserted T-DNA. The deduced protein shows no homology with any known proteins. These results strongly suggest that transcriptional activation of this novel gene causes the Sg1 phenotype. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project MP-1203).
植物化学調節学会の論文
2004-10-13

78. イネアクチベーションタギングにより単離された擬似病斑変異体及び短粒半わい性変異体の解析

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