31 ジャスモン酸結合タンパク質の単離・解析に向けたプローブの調製と生理活性

概要

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Jasmonic acid (JA) plays important roles in production of secondary metabolites including phytoalexins in suspension-cultured cells of rice, soybean, and many other plantS. As the first step of our study to isolate JA-binding proteins from the rice and soybean cells, we have prepared chemical probes that are useful for isolation and functional analysis of JA-binding proteins. In this studY, JA-amino acid conjugates (JA-Gly, (+)-JA-(S)-lle, (-)-JA-(S)--lle), JA-FITC conjugate, and JA-biotin conjugates were prepared by the active ester method, and their biological activities were determined by micro-drop method using rice seedlings. JA- amino acid conjugates showed almost the same growth inhibitory activities as JA. JA-FITC conjugate and JA-biotin conjugates were almost inactive. The effects of the JA analogues on production of phytoalexins in rice plants and on induction of phenylalanine ammonia-lyase (PAL) in suspension-cultured soybean cells will also be reported.
植物化学調節学会の論文
2001-10-09