32 イネにおけるジャスモン酸応答性遺伝子RERJ1の機能解析

概要

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Jasmonic acid (JA) is a plant hormone which regulates various developmental processes in plants, and it acts as an signal molecule in plant defense against herbivores and pathogens. We reported JA is a key signal transducer in the elicitor (N-acethylchitoheptaose)-induced phytoalexin production in suspension-cultured rice cells. To obtain a clue to unravel the signal transduction pathways of JA, we attempted cloning and chractarization of cDNAs for JA-responsive genes by differential screening of a cDNA library constructed using poly(A)^+mRNA from suspension-cultured rice cells treated with JA for 30 min, resulting in isolation of a cDNA clone tentatively named cRERJ1 that responded to exogenous JA by increase in the mRNA level. Northern blot analysis indicated that RERJ1 was early responsive to JA, the maximum level of RERJ1 mRNA being observed 30 min after the start of JA treatment. Based on the sequence analysis, RERJ1 is deduced to encode a transcriptional regulator having a basic region/helix-loop-helix motif. According to analysis of phenotypes of transgenic rice plants overexpressing sense- or antisense-RERJ1, it was strongly suggested that RERJ1 plays key roles in various jasmonic acid-mediated, biological phenomena such as inhibition of vegetative growth and induction of necrosis in the leaves. Investigetion of effects of JA on phytoalexin production in the transgenic rice plants is now under way.
植物化学調節学会の論文
2001-10-09