43. イネにおけるエリシター応答性 WRKY 遺伝子のクローニング

概要

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WRKY proteins are a large family of transcription factors that specifically bind to the DNA sequence motif TTGAC (C/T) (W-box). Many WRKY proteins have been suggested to have a regulatory function in the response to pathogen infection and other stresses. In suspension-cultured rice cells, diterpenoid phytoalexins are produced in response to exogenously applied elicitors such as chitin oligomers and cerebrosides from the pathogen Magnaporthe grisea. By means of microarray analysis of 8987 randomly selected rice expressed sequence tags (EST), we isolated two rice WRKY genes, OsSPF1 and OsABF2,that were induced after treatment with a chitin elicitor, N-acetylchitooctaose, in suspension-cultured rice cells. OsSPF1 and OsABF2 cDNAs encoding the full-length ORF were isolated by RACE-PCR with the primers designed on the basis of EST sequences. OsSPF1 belongs to groupI WRKY proteins (two WRKY domains) and OsABF2 belongs to group II WRKY proteins (one WRKY domain that contains the Cys_2His_2 zinc-finger-like motif). Transcripts og both genes in suspension-cultured rice cells began to accumulate 15 min after addition of the elicitor and reached a maximum level after 30 min, and then decreased gradually.
植物化学調節学会の論文
2003-10-10