81. イネにおけるジテルペン型ファイトアレキシン生産を制御するbZIP型転写因子OsTGAP1の機能解析(口頭発表,植物化学調節学会第46回大会)

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Momilactones and phytocassanes are major diterpenoid phytoalexins in rice. We showed that biosynthetic genes for momilactones and phytocassanes are respectively clustered on rice chromosome 4 and chromosome 2, and that an elicitor-inducible bZIP transcription factor, OsTGAP1, is involved in the coordinated regulation of the expression of biosynthetic genes for diterpenoid phytoalexins. Here we performed chromatin immunoprecipitation with next-generation sequencing technology (ChIP-seq) to elucidate the direct target genes of OsTGAP1. As a result, 4600 and 3793 binding sites were identified under the condition with and without elicitor treatment, respectively. There were 1843 and 1498 genes in which OsTGAP1 binding sites are present within 2 kbp from their transcription initiation sites under the condition with and without elicitor treatment, respectively. According to our previous transcriptome analysis, in these 1843 genes, 211 genes were up-regulated and 110 genes were down-regulated in OsTGAP1 over-expressing cells compared to wild-type cells. As for 1498 genes, 173 genes were up-regulated and 84 genes were down-regulated. We consider that these genes are the direct target genes of OsTGAP1. We also found that OsTGAP1 predominantly bound to the inetergenic regions and both ends of phytoalexin biosynthetic gene cluster and didn't bind to the upstream regions of the majority of biosynthetic genes for diterpenoid phytoalexins.
2011-10-03