30.イネのジャスモン酸応答性転写因子RERJ1に発現制御されるchitinase遺伝子のプロモーター解析(口頭発表)

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Jasmonic acid (JA) is a plant hormone that acts as a secondary signaling molecule in plant defense responses. RERJ1 that encodes a bHLH transcription factor was isolated as a JA-responsive gene from rice suspension cells. Previously, we reported that RERJ1 localized nuclear and functions as a transcriptional activator, and that a rice chitinase gene was screened as a RERJ1-taraget gene by the microarray analysis using RERJ1 overexpressor. The chitinase mRNA levels peaked at 4-6 h after JA treatment in rice cells. Besides, the expression of chitinase gene was induced by in a RERJ1-dependent manner in the transgenic rice in which RERJ1 expression could be controlled by an estrogen treatment. Here, we identified JA-responsive cis element in the promoter region of the chitinase by reporter-gene assay. We conducted the deletion analysis of the chitinase promoter regions located from -2415 to -265 bp upstream of the translation start site. Deletions from -2415 to -515 bp did not have a significant effect on responsiveness to JA, but a further deletion to -265 bp rendered the promoter incapable of responding to JA. This result suggests that the region from -515 to -265 bp contains JA-responsive cis element. Indeed, the region contains three E-box elements, which are putative binding sites for bHLH transcription factors. The mutation analysis showed that an E-box at -448 to -443 bp is essential for responding to JA. Direct binding of RERJ1 to the E-box is now being examined.
植物化学調節学会の論文
2007-10-05

30.イネのジャスモン酸応答性転写因子RERJ1に発現制御されるchitinase遺伝子のプロモーター解析(口頭発表)

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