58.イネのosjar1変異体のキャラクタリゼーション(口頭発表)

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Jasmonoyl-isoleucine (JA-Ile), which is biosynthesized by JAR, was reported to be a key molecule for COI1 mediated JA signaling pathway. In rice, it has been reported that OsJAR1 is involved in fertility and photomorphogenesis by characterizing rice osjar1 Tos17 mutant. However function of OsJAR1 in JA signaling pathway remains to be defined. In this study, we investigated some JA responsible phenotypes in osjar1 to understand the role of JA-Ile in rice. First we examined production of jasmonates. In wounded osjar1 leaves, accumulation of JA-Ile was severely repressed compared with wild-type rice, whereas accumulation of JA was comparable to wild-type rice. This suppression was complemented by expressing wild-type OsJAR1. These results strongly suggest that OsJAR1 is involved in JA-Ile biosynthesis in rice. Next we performed expression analysis of JA inducible genes in osjar1 mutant. The expressions of the induced monoterpenoid volatile linalool synthase gene, OsLS, the JA-induced bHLH type transcription factor gene, RERJ1, and JAZ genes after wounding or JA treatment were suppressed in osjar1. We further examined effects of JA and JA-Ile on accumulaton of phytoalexins in osjar1 leaves. JA-induced accumulation of phytoalexins seen in wild-type plant was not observed in osjar1 mutant. This suppression was recovered by JA-Ile treatment to osjar1. These results suggest that JA-Ile is essential for JA-induced gene expression and phytoalexins production.
2010-10-01

58.イネのosjar1変異体のキャラクタリゼーション(口頭発表)

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