26 植物共生菌 Azospirillum lipoferum FS におけるオーキシン生合成の制御機構

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Free-living plant growth-promoting rhizobacteria (PGPR) of the genus Azospirillum are poteitially applicable as biofertilizers, due to their ability to excrete phytohormones such as indole-3-acetic acid (IAA), gibberellins and cytokinins. IAA produced by PGPR probably plays an essential role in the plant-microbe interactions. However, regulatory mechanisms for IAA production in the bacteria have not yet beeen identified. We have already isolated the ipdc gene coding for indole-3-pyruvic acid decarboxylase (IPDC), a key enzyme in the indole-3-pyruvic acid pathway for IAA biosynthesis. We have also indicated that two DNA-binding proteins exist that might be involved in regulation of the ipdc expression in A. lipoferum FS, and that the DNA-binding of one protein is inhibited by auxin. In this study, we constructed a ipdc-gus fusion plasmid, and introduced the plasmid to A. lipoferum FS by electropolation. By the expression study using a transformant, we showed that expression of the ipdc gene is induced by exogenous IAA. We have also attempted to isolate two proteins that specifically bind to the sequence containing an inverted repeat sequence (IRS) in the upstream region of the ipdc gene. We performed several procedures for protein purification and identified a possible candidate for the protein of which DNA-binding is inhibited by auxin on SDS-PAGE. Now, we are trying to isolate the gene coding for the DNA-binding protein based on information from amino acid sequence.
植物化学調節学会の論文
2002-10-31

26 植物共生菌 Azospirillum lipoferum FS におけるオーキシン生合成の制御機構

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