D-42 アズキ黄化芽生え中に含まれるジベレリン結合タンパク質の精製

概要

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The perception of the specific molecule is the first step in the signal transduction of plant hormones leading to the regulation of physiological phenomena observed in the life cycle of higher plants. The induction of α-amylase in cereal grains and promotion of shoot elongation of higher plants are typical physiological events induced by gibberellin (GA). The signal transduction pathway of GA has been studied mainly in the α-amylase system which showed the localization of GA-receptor on plasma membrane. However, little is known about the GA perception in shoot elongation systems. We successfully detected in cytosolic fractions from etiolated azuki bean seedlings the protein capable of binding active GAs selectively, and partially purified this GA-binding protein (GBP). The apparent Kd value for GA_4, one of the active Gas, was 6 X 10-^<10> (M) by Scatchard plots analyses. The molecular size of the GBP was estimated to be 25 kDa by HPLC with a gel filtration column. We assume this GBP to be one of the receptor candidates and are further purifying it with various chromatographic methods.
植物化学調節学会の論文
1998-10-01