82 アサガオ未熟種子中で発現する GA 生合成酵素遺伝子のクローニングおよび発現解析

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Immature seeds of some dicotyledonous plants contain a large amount of gibberellins (GAs). Their physiological roles, however, remain unknown. We have reported the localization of active GAs and GA-inducible α-amylase (PnAmy1) around starch grains in integument of immature seeds of P. nil. The result suggests that the degradation of the starch by the induction of PnAmy1 may be one of the important roles of GAs in the immature seeds. To obtain further information on GAs in the seeds, we focused our efforts on the isolation of gibberellin biosynthesis genes, especially 20-oxidase, 3-oxidase, 2-oxidase, functioning in immature seeds of P. nil, and their expression analyses. Using the cDNAs derived from developing seeds and the degenerate primers which were designed based on the well conserved sequences in known gibberellin biosynthesis genes, we have amplified partial DNA fragments of two 20-oxidase genes (PnGA20ox1,2), two 3-oxidase genes (PnGA3ox1,2), one 2-oxidase gene (PnGA2ox1). We obtained full-length cDNAs for all five genes by 5'- and 3'-RACE. In situ hybridization experiments showed that both PnAmy1 mRNA and PnGA2ox1 mRNA localized at around seed coat adjacent to integument.
植物化学調節学会の論文
2002-10-31