75 アサガオ未熟種子中に含まれるα-アミラーゼ遺伝子の探索および発現解析

概要

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Immature seeds of dicotyledonous plants often contain a large amount of gibberellins(GAs). However their physiological roles remain unknown. By immunohistochemical analysis, we have reported the localization of GA1/3 at an opposite side of the hilum on starch grains in outer-integument of immature seeds of Pharbitis nil. This observation makes us associate that GAs may be concerned with the degradation of starch via an induction of specific hydrolytic enzymes like α-amylase as in germinating cereal grains. We focused our efforts on the isolation of α-amylase genes functioning in the seeds of P.nil, and their expression analyses. Using the cDNAs prepared from the immature seeds as template, we amplified partial DNA fragments by PCR with degenerate primers which were designed based on the well conserverd sequences in known α-amylase genes. We obtained two full-length cDNA(PnAmy1, 2) by 5'-and3'-RACE. The preliminary results of northern-hybridization analysis showed the tendency that these genes responded to active GA.
植物化学調節学会の論文
1999-10-01