76 シロイヌナズナにおけるゲノムDNA/cDNA発現ライブラリーの作製とスクリーニング

概要

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To establish a new system for functional genomics, a genomic and a cDNA expression libraries were prepared from Arabidopsis in a binary vector, pEXplant, newly constructed based on pBI121. The vector has a multi cloning site for cloning genomic DNA fragments or cDNAs downstream of CaMV 35S promoter. Altered phenotypes are anticipated by suppression of endogenous gene expression through overexpresion of antisense RNA or incomplete sense RNA, or by overexpression of sense RNA from sense-oriented cDNA, under the control of CaMV 35S promoter. Until now, we have harvested seeds of the second generation from approximately 1000 Arabidopsis transformants with genomic DNA fragments. Southern blot analysis and PCR indicated that independent genomic fragments were introduced into transformant genomes as T-DNAs from pEXplant. The sequence analysis of insert fragments and the change of the transcript levels will be performed to confirm the system works effectively.
植物化学調節学会の論文
1999-10-01