37. ストレス誘導性レセプター様キナーゼの下流で機能する因子のスクリーニング

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We screened Arabidopsis plants transformed with pSKI015 and obtained a mutant, 90-2. In 90-2,T-DNA was inserted in a receptor-like kinase (90-2-RLK) gene. Green fluorescence protein (GFP) fused to the 90-2-RLK protein was strongly detected in root apices, lateral root primordia, while weakly in procambium and pericycle cells. In root tissues, expression pattern of 90-2-RLK pro-GUS was identical to that of 90-2-RLK pro-90-2-RLK : : GFP. In aerial parts, GUS activity was localized in stipules and floral organ abscission zones. In leaves and stems, GUS activity was not detected but induced by wounding, salt and osmotic stress. To identify components of 90-2-RLK mediated signal transduction pathway in plants, we performed yeast two-hybrid screening of Arabidopsis cDNA library using the kinase domain of 90-2-RLK as a bait. 20 clones were obtained as truly positive clones that activate His3 and lacZ reporter genes reproducibly. Sequence analysis revealed that seven encoded a protein with unknown function, three encoded a protein containing tetratricopeptide repeat (TPR) domain, and two encoded a vacuolar sorting protein 35 homolog. The former two proteins showed interaction in yeast two-hybrid system even with the enzymatically inactive form of the kinase domain of 90-2-RLK as a bait. This result suggests that these proteins are not phosphorylation substrates but interacting partners of 90-2-RLK.
植物化学調節学会の論文
2003-10-10