18.抗活性型ジベレリン抗体の抗メタタイプペプチドのスクリーニング(口頭発表)

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An antibody (Ab) which recognizes another Ab binding with its ligand is called an anti-metatype Ab. Only limited numbers of anti-metatype Abs have been reported. In this study, we screened a phage display Ab library to obtain anti-metatype Ab of antibioactive gibberellin (GA) Ab. We also screened phage display peptide libraries to obtain peptides which bind anti-GA Ab in the GA dependent manner (tentatively designated as anti-metatype peptide). We screened anti-metatype Abs or peptides from a phage display single-chain variable-fragment (scFv) library and three kinds of phage display peptide libraries against anti-bioactive GA antibody (8/E9) in the presence of GA1 or GA4. Although anti-metatype scFv could not be obtained because of extensive non-specific and GA-non-dependent binding, two different anti-metatype peptides which bind to 8/E9 Ab in the presence of GA4 were obtained (designated as A peptide and I peptide). The I peptide, which showed clearer GA4 dependency for the binding, did not bind to 8/E9 in the presence of GA1, another ligand of 8/E9, suggesting that the structure of GA4 molecule directly participated in the binding. I peptide was not reactive with 21/D13, another anti-bioactive GA antibody, regardless of GA existence, indicating that both GA4 and the partial structure of the 8/E9 are recognized by the peptide. Using 8/E9 and I peptide, an ELISA system equivalent to sandwich ELISA was established to detect as low as 30 pg of GA4 specifically.
植物化学調節学会の論文
2007-10-05

18.抗活性型ジベレリン抗体の抗メタタイプペプチドのスクリーニング(口頭発表)

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