73. シロイヌナズナの花茎で強く発現するアラビノガラクタンタンパク質(AtFLA11)の機能解析

概要

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Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Little is known about the precise function(s) of any single AGP, but they are implicated in diverse developmental roles. Now that the genome of Arabidopsis is sequenced, it is theoretically possible to identify all of the AGP protein backbone genes. We prepared an AGP macroarray by cloning gene fragments of all the Arabidopsis AGPs as a handy detection system of AGP gene expression. Using this system, the expression patterns of AGPs in various tissue types were analysed. AtFLA11 was expressed at higher level than any other AGP gene in inflorescense stem. An atfla11 knockout line did not show any distinguishable phenotype from WT under the normal growth condition. Northern analysis has shown that AtFLA11 expression is localized in inflorescense stems and siliques. AtFLA12 mRNA which shows highest homology with AtFLA11 showed a similar expression pattern to AtFLA11, suggesting that AtFLA12 may have the redundant functions with AtFLA11 in planta. In situ hybridization experiments showed that AtFLA11 mRNA is localized in sclerenchyma cells such as fiber cells and xylem cells. We are now examining the possibility that AtFLA11 is involved in the secondary wall development in sclerenchyma cells by detail analysis of the atfla11 knockout line.
植物化学調節学会の論文
2004-10-13