30.ジベレリン受容体解析プローブの創製 : ジベレリン誘導体の調製と受容体結合性に関する構造活性相関(口頭発表)

概要

論文の詳細を見る
Introduction Gibberellin (GA) is one of the phytohormones that promotes seed germination, stem elongation and flower development, and GA receptor GID1 was identified in 2005. We prepared C16, 17-modified gibberellin A4 derivatives and evaluated their binding activity to GID1 to develop a molecular probe for the analysis of GA-GID1 interaction. Methods and Results We modified C16, 17 of GA molecule by introduction of alkyl chain or phenyl group through C-C or C-O bond. First we tested these GA derivatives with yeast 2-hybrid system in which only active GA (ex. GA_1, GA_4 etc.) can induce GID1-DELLA interaction. Then we evaluated their in vitro binding activity with inhibition of tritium labeled GA-GID1 interaction. Both introduction of phenyl group and connection of alkyl group through C-O bond drastically decreased the activity. While the introduction of alkyl group through C-C bond still kept the activity. On the basis of the structure-activity relationship study, we found that both steric hinderance and electronic distribution around C16, 17 moiety of GA molecule affect binding activity.
2008-10-06