65 抗chlorpropham一本鎖抗体を発現した植物の調製とその応用

概要

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In recent years, many reports have described expression of antibodies or antibody fragments in plants for various applications. We constructed a single-chain Fv (scFv) against chlorpropham, a preemergent herbicide, from the monoclonal antibody (mAb) producing hybridoma cell, K2158. The scFv showed the binding activity by ELISA. The binding affinity of the scFv was lower than that of the original mAb, but still high enough, and the scFv showed the similar binding specificity to the mAb. We introduced the scFv gene into tobacco. The expression vectors were designed to target the scFv into ER, cytoplasm or apoplastic space. The vectors for the expression of the scFv as a glycosyl-phosphatidyiinositol (GPI) anchored protein were also designed. So far, some transgenic plants have regenerated. We will analyze these plants to confirm the integration of the transferred gene and determine the expression level.
植物化学調節学会の論文
2001-10-09