Molecular Cloning and Expression in Saccharomyces cerevisiae of Tobacco NADPH-Cytochrome P450 Oxidoreductase cDNA

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概要

• 論文の詳細を見る

• We obtained information on the full length tobacco NADPH-cytochrome P450 oxidoreductase (P450 reductase) by a combination of the cDNA clone pCTR1 and the genomic DNA clone pGTR1. The deduced primary structure consisting of 713 amino acid residues contained sequences corresponding to FMN, FAD, and NADPH-binding regions. Based on this information, we prepared the full-length cDNA pFTR of tobacco P450 reductase by RTPCR and expressed it in the yeast Saccharomyces cerevisiae. The transformed yeast cells carrying pFTR produced the corresponding mRNA and protein, and had increased cytochrome c reductase activity in the microsomes. An in vitro reconstitution system of the yeast microsomal fractions expressed tobacco P450 reductase and rat P450 1A1 showed an increased 7-ethoxycoumarin O-deethylase activity. These results indicated that tobacco P450 reductase expressed in the yeast microsomes coupled with rat P450 1A1 resulting in an increased monooxygenase activity.

• 社団法人日本農芸化学会の論文
• 1998-07-23

著者

• Yamada Takashi

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• 大川 秀郎

  神戸大学農学部

• Inui H

  Research Center For Environmental Genomics Kobe University

• 大川 秀郎

  神戸大・自然科学:遺伝子実験センター:(現)福山大・グリーンサイエンス研究センター

• OHKAWA HIDEO

  Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University

• Yamada Takashi

  Department Of Chemistry Faculty Of Science And Engineering Konan University

• 今石 浩正

  Research Center For Environmental Genomics Kobe University

• IMAISHI Hiromasa

  Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University

• OKA Atsuhiro

  Laboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University

• Oka Atsuhiro

  Laboratory Of Molecular Biology Institute For Chemical Research Kyoto University

• Ohkawa Hideo

  Deparment Of Biological And Environmental Science Faculty Of Agriculture Kobe University

• Imaishi Hiromasa

  Department Of Biological And Enviromental Science Faculty Of Agriculture Kobe University

• Yamada T

  National Institute Of Agrobiological Sciences Ministry Of Agriculture Forestry And Fisheries

• Yamada Takashi

  Department Of Animal Science Graduate School Of Agricultural Science Kobe University

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