3 キュウリ胚軸におけるジベレリン応答性遺伝子の検索

概要

論文の詳細を見る
Shoot elongation is one of the most obvious phenomena on treatment with gibberellin (GA). Intact cucumber seedlings were used for bioassay of the growth promoting activity of GA. Thus, we attempted to clone the GA-responsive genes from cucumber hypocotyls to obtain basic information on the regulatory mechanisms for shoot elongation using fluorescent differential display(FDD). FDD is a powerful method for identifying genes that are up-or down-regulated by stimuli. Two days after treatment with uniconazole-P, GA-synthesis inhibitor, cucumber seeds were sown and grown in vermiculite for 5 days. The cDNAs were synthesized with total RNAs prepared from cucumber hypocotyls 1 and 3 h after treatment with or without GA_4, and they were used as the templates for the polymerase chain reaction (PCR). The PCR products were run on polyacrylamide gels and the bands that correspond to differentially-expressed mRNAs were excised from the gel. We are examining the expression of these genes by Northern blot analysis.
植物化学調節学会の論文
1999-10-01