29 光によるブラシノステロイド6位酸化酵素の制御

概要

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Light has been suggested to be a negative regulator of brassinosteroid biosynthesis, but there have been no direct evidences. We analyzed light regulation of brassinosteroid biosynthesis both from biochemical and molecular biological approach. We first isolated brassinosteroid-6-oxidase gene (B6OX) from Arabidopsis. Total RNAs were extracted from Arabidopsis seedlings grown in light or darkness for 5 to 7 days. They were then subjected to realtime monitoring RT-PCR. Transcript level of B6OX gene was higher in plants grown in darkness than in light. We also performed feeding experiment of [^2H_6]6-deoxoCS using light- and dark-grown cucumber and Arabidopsis seedlings. Although we successfully detected [^2H_6]CS from both cucumber and Arabidopsis, significant differences were not observed between light- and dark-grown seedlings.
植物化学調節学会の論文
2000-11-02