ビチオン化アブシジン酸を用いた気孔孔辺細胞のABA結合部位の可視化

概要

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Abscisic acid (ABA) has been shown as a stress signal to regulate plant water relations under drought by closing stomata. Though ion channel regulation by ABA in guard cells have been extensively studied as a model system of plant signal transduction [1,2], molecular identification as well as celluar localization of ABA receptor(s) are not clarified. Using biotin-labeled ABA (Bio-ABA), we examined the ABA binding sites in stomatal guard cells of Vicia fava and Arabidopsis thaliana. Bio-ABA had slightly weaker but similar effects on stomatal closing and shrinking of guard cell protoplasts. ABA binding sites were visualized by fluorescence microscopy and also analyzed by flow cytometry using Bio-ABA and Alexa Fluor 488-labeled avidin. Fluorescent particles were observed on the surface of guard cell protoplasts in patches, suggesting the presence of ABA binding sites on the plasma membrane of stomatal guard cells.
植物化学調節学会の論文
2000-11-02