アリューロン細胞におけるフォスファチジルイノシトール-3-カイネースの作用

概要

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Aleurone cells undergo cell death in germination. Phosphatidylinosito1-3-kinase (PI3K) is associated with celldeath in animals, but relations between cell death and PI3K is till unknown in plants. We examined a role of PI3K in cell death in barley aleurone protoplast using PI3K specific inhibitor, wortmannin (WT) or putative PI3K product, phosphatidylinositol-3-phosphate (PI3P). As a result, GA or WT induced cell death and GA+WT induced cell death synergistically. ABA inhibited GA-induced cell death, but not WT-induced cell death. Addition of PI3P retarded WT-induced cell death, but not GA-induced cell death. These results suggest that PI3K might be associated with cell death signaling pathway, which could be different from GA-induced cell death signaling pathway. In GA-induced α-amylase synthesis and secretion test, WT inhibited GA-induced α-amylase activity whereas coapplication of PI3P with GA and WT canceled the inhibitory effect of WT. These results suggest that PI3K might be associated with GA-induced α-amylase signaling in barley aleurone cells.
植物化学調節学会の論文
2000-11-02