22 タバコ培養細胞BY-2におけるアミロプラスト分化誘導過程で発現の上昇する遺伝子群の単離

概要

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In cultured Bright Yellow-2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4- dichlorophenoxyacetic acid (2,4-D) in the culture medium induces amyloplast development. This differentiation also includes a decrease in cell multiplication, and an increase in cell size. These changes were primarily triggered by the depletion of 2,4-D, and accelerated by the addition of benzyladenine. Thus, this simple in vitro amyloplast-inducing system provides an opportunity to analyze and compare heterotrophic cells in meristematic and starch-storing phases. In order to analyze the mechanisms controlling starch-storing cell differentiation, we started to isolate genes specifically expressed during amyloplast differentiation. Using differential display method, we cloned six cDNA fragments whose transcripts accumulate at high levels during starch-storing cell differentiation. Three clones had homology to known genes;i.e. clone 26, 30, and 60 had significant identities with cellulose synthase, glutamate dehydrogenase, and malate dehydrogenase, respectively. Other three clones (clone 10, 22, and 25) had no significant identities with well-characterized genes, while sequence similarity search using BLAST algorithm suggested that they are homologous to Arabidopsis genes of unknown functions. Analyses on these genes are now in progress.
植物化学調節学会の論文
2001-10-09