18 アブシジン酸による発現増幅を利用したアサガオ花成特異的遺伝子の単離

概要

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Possible roles of abscisic acid (ABA) in photoperiodic flowering of Pharbitis nil was examined and then we tried to isolate flowering-specific genes of which expression was amplified by the treatment with ABA. There were no remarkable differences in endogenous ABA levels in the cotyledons between under short-day and long-day conditions. Effect of fluridone, an inhibitor of ABA biosyntheses, on flowering was only small. These results indicate that endogenous ABA does not play an important role in the photoperiodic flowering. Flower-promoting effect of exogenous ABA was lost when ABA was applied to shoot apex 24h after the end of a dark treatment. Thus, mRNA were extracted from shoot apices 24h after the end of a 16h darkness. mRNAs extracted from plants treated with a 16h darkness and from those with a 16h darkness plus a night break were used for colony hybridization to isolate dark treatment specific genes. Genes of which expressions areamplified by ABA treatment can be identified as flowering-specific genes through northern blot analysis using cDNAs for dark treatment-specific genes as probes.
植物化学調節学会の論文
1999-10-01