E-46 タバコ緑色培養細胞におけるサイトカイニン誘導性遺伝子の解析

概要

論文の詳細を見る
Suspension cultured green cells of tobacco (Nicotiana tabacum cv. Samsun NN) requires exogenous cytokinin and auxin in the mediun for growth. The tobacco cells were cultured in cytokinin-starved maedium 4 days, then bach to normal cytokinin and auxin medium.The cytokinin stimulated tobacco cells showed higher chlorophyll synthesis, higher growth and higher expression of chloroplastic genes than continuous cytokinin-starved cells after 1 or 2 weeks cultures. To identify mRNA expressed and/or related in early stage of cytokinin signal transductions, 4 cDNA fragment of cig (cytokinin inducible gene) were isolated from cylokinin stimulated tobacco cells after 12 and24 hr stimulation by fluorescent differential display. Sequencing analysis could suggest characteristic structures and possible homologues of cig
植物化学調節学会の論文
1998-10-01