13 HMG-CoA Reductaseの翻訳後修飾に関わるキナーゼ遺伝子の探索

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In higher plants, isoprenoids are synthesized via mevalonate pathway and non-mevalonate pathway. Sterols are formed in cytoplasm via the former pathway, whereas chlorophyll and carotenoids are synthesized in plastids via the latter pathway. The key enzyme on mevalonate pathway is 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). HMGR is regulated on the transcriptional and post-translational level. HMGR is inactivated by AMP-activated protein kinase (AMPK) in mammals. In plants, however, it is not known whether HMGR is regulated by AMPK-homologous kinases (SnRKs) in vivo. Therefore, we analyzed Arabidopsis SnRKs, Akin10,Akin11 and Akin12. To determine organs where Akin10,Akin11 and Akin12 are expressed, we examined the RT-PCR analysis. Akin10 and Akin 11 are expressed ubiquitously. On the other hand, Akin12 is expressed in immature siliqs. Plant SnRKs can be divided into two groups according to their expression profiles; the one group expressing ubiquitously and another group expressing in endosperm. All genes belonging to the latter group were isolated from monocots. Akin12 may be the first gene belonging the latter group isolated from dicots. Akin12 is transcribed to two kinds of mRNA by alternative splicing. The putative larger polypeptide (Akin12(L)) contains kinase domain in the N-terminal half and the regulatory domain in the C-terminal end, and the putative smaller polypeptide (Akin12(S)) lacks the C-terminal regulatory domain. Therefore, Akin12(S) may be constitutive active. Sequence analysis show the possibility that intracellular localization of Akin10,Akin11 and Akin12 is different from each other. The role of the each kinase is discussed from the viewpoint of expression profile and intracellular localization.
植物化学調節学会の論文
2002-10-31

13 HMG-CoA Reductaseの翻訳後修飾に関わるキナーゼ遺伝子の探索

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