45 LC-APCI/MS/MSによる内生サイトカイニン一斉分析法

概要

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Newly rapid determination methods for natural cytokinins in the plant was developed by LC-APCI/MS/MS. We separated and determinated 9 natural cytokinins and cytokinin derivatives. Mobile phase constituted 0.01M Ammonium hydrogen carbonate and 13%(v/v) acetonitrile. An analysis was done about the solvent which 80% extracted by the mothaol solvent from plants. And we used d_5-Zeatin for the internal standard, and a good straight line was shown about trans-cis-zeatin (t-Z, c-Z), trans- and cis-zeatin riboside (t-ZR, c-ZR), isopentenyladenin(iPA), isopentenyladenin riboside monophosphate(iPAMP), t-zeatin riboside monophosphate(ZRMP), t-zeatin-O-glucoside(ZoG) and t-zeatin-O-glucoside riboside(ZoGR), in the absolute quantity in the range of 10 pg from 1000 pg. These limit of detection (S/N=3) was about 3 pg (t-Z).
植物化学調節学会の論文
2001-10-09