64.シロイヌナズナにおける9-hydroxy-10-oxo-12(Z),15(Z)-octadecandienoic acid(KODA)生合成遺伝子LOXの機能解析(口頭発表)

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Novel Oxylipin, 9-hydroxy-10-oxo-(Z), 15(Z)-octadecadienoic acid (KODA) is one of signal compounds, which is synthesized from the linolenic acid. It was reported that KODA has a function to enhance stress tolerance and to activate inflorescence development in some species. It has been suggested that KODA biosynthesis involve two kinds of enzymes (LOX, AOS) as in the case of Jasmonate (JA). It has also suggested that there are some differences in substrate specificity of enzyme activity between biosynthesis of KODA and JA. Namely 9-specific oxidation enzymes were necessary in KODA biosynthesis, while 13-specific enzymes in JA. According to a recent study, 9-specific enzyme accumulates tissue-specifically in vascular bundle of Solanum lycopersicom, and Solanum tuberosum. However there was little information about 9-specific enzyme in detail. In Arabidopsis thaliana the expression of one AOS and 6 LOXs were detected, but their involvement in KODA biosynthesis remained to be elucidated. To clarify 9-specific enzyme activity for KODA production, we have established that loss of function or overexpression mutants of six LOXs. Differences were observed in leaf enlargement and inflorescence elongation. To clear whether the levels of endogenous KODA were regulated by the expression of LOXs or not, we established GC/MS analysis method. Preliminary analysis to detect the levels of endogenous oxylipins in Arabidopsis were performed using by this method.
植物化学調節学会の論文
2008-10-06

64.シロイヌナズナにおける9-hydroxy-10-oxo-12(Z),15(Z)-octadecandienoic acid(KODA)生合成遺伝子LOXの機能解析(口頭発表)

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