26. カンゾウ(Glycyrrhiza uralensis)におけるグリチルリチンの生合成中間体および生合成関連遺伝子の探索

概要

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Glycyrrhizin, a sweet triterpene saponin of Glycyrrhiza uralensis, exhibits various pharmaceutical effects. To produce a better quality of pharmacologically active G. uralensis plant by metabolic engineering approach, it is necessary to understand the regulatory mechanism of glycyrrhizin biosynthesis. For this purpose, we are investigating intermediates and candidates for biosynthetic genes for glycyrrhizin biosynthesis. Since glycyrrhizin should be biosynthesized via β-amyrin by a series of oxidation and glycosylation, several oxygenated and/or glycosylated β-amyrin derivatives are examined as the putative biosynthetic intermediates. The stolon of G. uralensis was analyzed by GC-MS and LC-MS using authentic samples prepared by organic synthesis. All the putative biosynthetic intermediates are detected, strongly supporting the proposed pathway. We genetated over 12,000 expressed sequence tags (ESTs) derived from stolon. Database search revealed that 27, 12 and 40 EST clones showed significant similarities to known P450, dioxygenase, and glucosyltransferase or glycosyltransferase sequences, respectively.
植物化学調節学会の論文
2005-10-13