84.ヨモギ属植物の新規セスキテルペン環化酵素遺伝子の単離(口頭発表)

概要

論文の詳細を見る
Artemisia spp. have been used for medicine and food. It is well known that A. annua contains an anti-malarial sesquiterpenoid, artemisinin. Therefore both of chemical and molecular genetic studies have been intensively performed on this species. However, there were few such studies of other Artemisia spp. Previous comparative sesquiterpenoid profiling of 33 Artemisia spp. revealed characteristic profile of A. abrotanum, in which silphiperfolane compounds occupied 60% of total sesquiterpenoid. This finding suggested that yet unidentified gene encoding silphiperfolane synthase was expressed in A. abrotanum. To isolate novel sesquiterpene cyclase gene(s) from A. abrotanum, RT-PCRs using degenerate primers and subsequent RACE-PCRs were performed. Four cDNA fragments potentially encoding a novel sesquiterpene cyclase, with 34-70% identity with A. annua sequiterpene cyclases (Fig. 1), were obtained. Functional analysis is currently in progress.
2010-10-01