59. シロイヌナズナラノステロール合成酵素の同定

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Sterols exist in cells of all organisms as components of cellular membranes and precursors of hormones. Sterols are cyclized from epoxy-squalene, however, the mechanism of cyclization differs between vertebrates and plants. While 2, 3-epoxysqualene is firstly cyclized to lanosterol in vertebrates, it has been reported that 2, 3-epoxysqualene is firstly cyclized to cycloartenol in plants. Since it has been also reported that lanosterol was identified in Euphorbia-lathyris, it is obscure whether all phytosterol are biosynthesized via cycloartenol. Genome sequence analyses suggested that two genes encoding cycloartenol synthase, At2g07050 (CAS1) and At3g45130, exist in the Arabidopsis genome. Although CAS1 was reported as functional cycloartenol synthase, At3g45130 is only considered to encode cycloartenol synthase by sequence homology. To identify the biochemical properties of At3g45130, we performed the complementation test of lanosterol synthase deficient yeast GIL77 with At3g45130 and analyzed the metabolite of the yeast. At3g45130 could complement the lanosterol synthase deficiency. The metabolite analyses of this yeast on TLC, GC-MS and NMR demonstrated that GIL77 expressing At3g45130 biosynthesize lanosterol. Next, as a first approach to clarify the role of At3g45130 in plant, the expression profile of At3g45130 was analyzed. Although the expression level of At3g45130 was much lower than that of CAS1, the expression of At3g45130 was detected in root, stem and siliques.
植物化学調節学会の論文
2005-10-13

59. シロイヌナズナラノステロール合成酵素の同定

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