35.ヨモギ属植物におけるセスキテルペノイドプロファイルの比較解析(口頭発表)

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Artemisia spp. have been used for medicine and food. Artemisia annua contains anti-malarial sesquiterpenoid artemisinin. Therefore intensive studies on both chemical and molecular genetics have been done in A. annua. However, there were few such studies in other Artemisia spp. We previously demonstrated that sesquiterpenoid profile was correlated with gene expression of sesquiterpene synthase among A. annua, A. afra, and A. absinthium, suggesting that comparative approach is useful to find new gene function. Toward systematical understanding of molecular basis for chemical differences among Artemisia spp., sesquiterpenoid profiling was conducted. In this work, leaves of 33 Artemisia spp. were collected and their sesquiterpenoid profile was analysed by GC-MS. Germacrane, copaene, and caryophyllane were commonly detected. In contrast, some spp. had characteristic sesquiterpenoid profiles. Based on sesquiterpenoid profiles, biosynthetic mechanism of each sesquiterpenoid was proposed. For sesquiterpenes commonly detected, genes for corresponding sesquiterpene cyclases should be commonly existed in Artemisia spp. On the other hand, novel sesquiterpene synthase genes should be expressed in the restricted species which showed characteristic sesquiterpenoid profile. A. abrotanum contained silphiperfolane compounds in major proportions. This suggests that yet unidentified gene encoding silphiperfolane synthase is highly expressed. Molecular cloning of this gene is currently in progress.
2009-10-06

35.ヨモギ属植物におけるセスキテルペノイドプロファイルの比較解析(口頭発表)

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