42. 新規緑化変異体 hayamidori (ham) の解析

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Brassinazole (Brz) is a specific inhibitor for brassinosteroid biosynthesis. The inhibitor is useful to examine functions of brassinosteroid. Our previous experiments demonstrated that Brz treatment to seedlings enhanced greening when they were transferred from dark to light. In this study, we tried to isolate mutants showing different greening phenotypes from T-DNA activation tag lines of Arabidopsis. Four thousands of T-DNA activation tag lines were screened under the Brz treated condition, and a hayamidori (ham) mutant was isolated. When seedlings were transferred from dark to light, the mutant showed fast greening phenotype compared with a wild type. After 7 hours-transfer to light, chlorophyll content of the mutant was twice higher than that of a wild type. ham mutant also showed a dwarf, reduced apical dominancy, and male sterile phenotypes. THese phenotypes were maintained only in heterozygote plant, which suggested that the mutant is dominant and that ham is caused by the activation of some genes by the enhancers on the inserted T-DNA region. To identify the insertion position of the T-DNA in the mutant, plasmid rescue was performed. As a result, the T-DNA was inserted in the 6th exon of a gene with unknown function on the chromosome 4. Expression of 8 genes near the inserted gene was examined by RT-PCR and found out that 3 genes including a putative Myb-type of transacting factor were activated in the mutant. We are now establishing transgenic plants that overproduce candidate genes to identify the genes responsible for ham mutation.
植物化学調節学会の論文
2003-10-10

42. 新規緑化変異体 hayamidori (ham) の解析

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