74 アサガオのclass1 knox 遺伝子、PKn1, 2, 3の発現および機能解析

概要

論文の詳細を見る
Pharbitis nil is a good material for studying the differentiation switching mechanism at shoot apex because of its strict photoperiodism for flower induction. Three Kotted1 homologs, PKn1, 2 and 3 from P.nil are investigated as candidates which might be involved in regulation of cell differentiation at shoot apical meristems(SAM). RT-PCR analysis showed that their transcript levels were high in stems, intermediate in shoot buds and roots, and there was nearly no expression in cotyledons. In situ hybridization and RT-PCR detected PKn3 transcript in juvenile leaf, while common class1 knox genes including PKn1 and PKn2 cannot be detected in differentiated organs. To examine the functional identity of PKns with other Knotted1 homologs, these genes were ectopically expressed in tobacco under the control of CaMV 35S promoter. PKn1 and PKn2 transformants exhibited clear morphological alterations, especially in leaves, suggesting they pay an important role in leaf morphogenesis. Tobacco overexpressing PKn3, however, did not show aberrant phenotype, suggesting its functional difference from common class1 knox genes.
1999-10-01